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  • 無菌室的基本要求有哪些?
    來源:http://www.nexregen.com 瀏覽: 發布日期:2021-06-08
    無菌室一個應用行業非常廣泛的基礎性配套產業,在電子信息、半導體、醫藥衛生、生物工程、航天航空等眾多行業均有應用,并根據行業的精 密與無塵要求,等級差別也較大。無菌室應根據既經濟又科學的原則來設置。其基本要求有以幾點:
    Aseptic room is a basic supporting industry widely used in electronic information, semiconductor, medical and health, bioengineering, aerospace and many other industries. According to the industry's precision and dust-free requirements, the grade difference is also large. Aseptic room should be set up according to the principle of economy and science. The basic requirements are as follows:
    一、無菌室要求
    1、 Aseptic room requirements
    1、無菌室應有內外兩間,內間是無菌室,外間是緩沖室。房間容積不宜過大,以便于空氣滅菌。小的內間面積2x2.5= 5m2 ,外間面積1x2= 2m2 ,高以2.5m以下為宜,都應有天花板。
    1. There should be two inside and outside aseptic rooms, the inside is aseptic room and the outside is buffer room. The room volume should not be too large for air sterilization. The small inner space area is 2x2.5 = 5m2, the outer space area is 1x2 = 2M2, the height should be less than 2.5m, and there should be ceiling.
    2、內間應當設拉門,以減少空氣的波動,門應設在離工作臺為遠的位置上。外間好也用拉門,要設在距內間遠的位置上。
    2. Sliding door should be set in the inner room to reduce the fluctuation of air, and the door should be set far away from the worktable. It's better to use sliding door in the outer room, which should be set far away from the inner room.
    3、在分隔內間與外間的墻壁或隔扇上開一個小窗,作接種過程中必要的內外傳遞物品的通道,以減少人員進出內間的次數,降低污染程度。小窗寬60cm、高40cm、 厚30cm,內外都掛對拉的窗扇。
    3. A small window is opened on the wall or partition fan separating the inner room from the outer room, which is used as the necessary passage for internal and external delivery of articles in the vaccination process, so as to reduce the number of people entering and leaving the inner room and reduce the degree of pollution. The small window is 60cm in width, 40cm in height and 30cm in thickness, and the inside and outside are hung with opposite sashes.
    4、無菌室容積小而嚴密,使用一段時間后,室內溫度很高,故應設置通氣窗。通氣窗應設在內室進門處的頂棚上(即離工作臺遠的位置),好為雙層結構,外層為百葉窗,內層可用抽板式窗扇。通氣窗可在內室使用后、滅菌前開啟,以流通空氣。有條件可安裝恒溫恒濕機。
    4. The volume of aseptic room is small and tight. After using for a period of time, the indoor temperature is very high, so ventilation window should be set. The ventilation window should be set on the ceiling at the entrance of the inner room (i.e. the position far away from the worktable). It should be of double-layer structure, the outer layer should be louver, and the inner layer can be plate type window sash. The ventilation window can be opened after use and before sterilization to circulate air. If possible, a constant temperature and humidity machine can be installed.
    二、無菌室內設備和用具
    2、 Sterile indoor equipment and appliances
    1、無菌室內的工作臺,不論是什么材質用途,都要求表面光滑和臺面水平。
    1. The worktable in aseptic room, no matter what material is used, requires smooth surface and level worktable.
    2、在內室和外室各安裝一個紫外燈 (多為30W )。內室的紫外線燈應安裝在經常工作的座位正上方離地面2m ,外室的紫外線燈可安裝在外室中 央。
    2. A UV lamp (mostly 30W) is installed in the inner room and the outer room respectively. The ultraviolet lamp in the inner room should be installed directly above the seat that works frequently, 2m above the ground, and the ultraviolet lamp in the outer room can be installed in the center of the outer room.
    3、外室應有專用的工作服、鞋、帽、口罩、盛有來蘇兒水的瓷鹽和毛巾、持噴霧器和5%石炭酸溶液等。
    3, the outer room should have special work clothes, shoes, caps, masks, porcelain salt and towel, spray and 5% carbolic acid solution.
    4、內室應有酒精燈、常用接種工具、不銹鋼制的刀、剪、鑷子、70%的酒精棉球、工業酒精、載玻璃片、特種蠟筆、記錄本、鉛筆、標簽紙、膠水、廢物筐等。
    4. There should be alcohol lamp, common inoculation tools, stainless steel knife, scissors, tweezers, 70% alcohol cotton ball, industrial alcohol, glass slide, special crayon, record book, pencil, label paper, glue, waste basket, etc.
                                           無菌室裝修
    三、無菌室的滅菌消毒
    3、 Sterilization of aseptic room
    1、蒸:這是無菌室徹 底滅菌的措施。無菌室使用了較長時間,污染此較嚴重時,應進行薰蒸滅菌??捎眉兹?、乳酸或硫磺薰蒸。
    1. Steaming: This is a measure of complete sterilization in the sterile room. When the aseptic room has been used for a long time and the pollution is serious, fumigation and sterilization should be carried out. It can be fumigated with formaldehyde, lactic acid or sulfur.
    2、噴霧:在每次使用無菌室前進行。噴霧可促使空氣中微粒及微生物沉降,防止桌面、地面上的微塵飛場,并有殺菌作用??捎?%石炭酸噴霧。
    2, spray: before each use of the asepsis room. Spray can cause particles and microorganisms to settle in the air, preventing dust flying on the desktop and ground, and having bactericidal effect. It can be sprayed with 5% carbolic acid.
    3、紫外線照射:在每次使用無菌室前進行。紫外線有較好的殺菌效果。通常應開啟紫外線燈照射30 ~ 60min。
    3. Ultraviolet radiation: before each use of sterile room. Ultraviolet has better germicidal efficacy. Generally, the ultraviolet lamp should be turned on for 30 ~ 60min.
    四、無菌室工作規程
    4、 Working procedure of aseptic room
    1、無菌室滅菌。每次使用前開啟紫外線燈照射30min以上,或在使用前30min ,對內外室用5%石炭酸噴霧。
    1. Sterile room sterilization. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
    2、用肥皂洗手后,把所需器材搬入外室。在外室換上已滅菌的工作服、工作帽和工作鞋,戴好口罩,然后用2%煤酚皂液將手浸洗2分鐘。
    2. After washing hands with soap, move the necessary equipment into the outside room. In the outer room, put on sterilized work clothes, work caps and work shoes, wear masks, and then soak hands with 2% coal phenol soap for 2 minutes.
    3、將各種需用物品搬進內室清點就位,用5%石炭酸在工作臺面上方和操作員站位空間噴霧,返回外室, 5~ 10min后再進內室工作。
    3. Move all kinds of articles into the inner room and place them in place. Spray the 5% carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5~ 10min.
    4、接種操作前,用70%酒精棉球擦手。進行無菌操作時,動作要輕緩,盡量減少空氣波動和地面揚塵。
    4. Before inoculation, wipe hands with 70% alcohol cotton ball. During aseptic operation, the movement should be gentle to minimize air fluctuation and dust on the ground.
    5、工作中應注意安全。如遇棉塞著火用濕布包裹熄滅,切勿用嘴吹,以免擴大燃燒。如遇有菌培養物灑落或打碎有菌容器時,應用浸潤5%石炭酸的抹布包裹后, 并用浸潤5%石炭酸的抹布擦拭臺面或地面,用酒精棉球擦手后再繼續操作。
    5. Pay attention to safety in work. If the cotton plug catches fire, wrap it with wet cloth and extinguish it. Do not blow it with your mouth to avoid expanding combustion. In case of bacteria culture spilling or breaking the bacteria container, wrap it with a cloth soaked with 5% carbolic acid, wipe the table or ground with a cloth soaked with 5% carbolic acid, and then continue the operation after wiping hands with alcohol cotton ball.
    6、工作結束后立即將臺面收拾干凈,將不應在無菌室存放的物品和廢棄物全部拿出無菌室后,對無菌室用5%石炭酸噴霧,或開紫外線燈照射30min。
    6, after the work is finished, clean up the table immediately, and remove all the articles and wastes that should not be stored in the asepsis room, then use the 5% carbolic acid spray or the ultraviolet lamp to irradiate 30min.
    以上就是無菌室的配置要求,更多實驗室相關資訊請關注我們。感謝您的閱讀,希望以上內容能對您有所幫助,如您想了解更多精彩內容請點擊我們的官網:無菌室裝修http://www.nexregen.com。
    The above is the configuration requirements of aseptic room. Please pay attention to us for more laboratory information. Thank you for reading, hope the above content can help you, if you want to know more wonderful content, please click our official website: aseptic room decoration http://www.nexregen.com .
    相關標簽: 空氣凈化工程、實驗室裝修、無菌室裝修、
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